Recombinant antibodies (rAbs) are monoclonal antibodies produced in vitro using synthetic genes. Unlike monoclonal antibodies (mAbs), which are produced using traditional hybridoma-based technology, rAbs do not require hybridomas and animals for their production.
Antibodies, both monoclonal and recombinant, can be used in biomedical science and toxicology research and are effective in the therapeutic treatment of cancer, autoimmune diseases, and many other diseases. To know more about recombinant antibodies, you can also browse https://www.bosterbio.com/featured-products.
However, while monoclonal antibodies have become one of the most common tools in biomedical sciences and medicine because of their ability to bind and neutralize or destroy cell-specific antigens, this method of producing ascites causes significant pain and discomfort in the animals used.
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Recombinant Antibody Production
In general, recombinant antibodies are monoclonal antibodies produced in vitro using synthetic genes. The technology involves obtaining antibody genes from source cells, amplifying and cloning the genes into appropriate phage vectors, inserting the vectors into the host (bacterial, yeast, or mammalian cell lines), and achieving adequate expression of functional antibody levels.
Recombinant antibodies can be cloned from any antibody-producing animal provided suitable oligonucleotide primers or hybridization probes are available.
The ability to manipulate antibody genes also makes it possible to generate new antibodies and antibody fragments (Fab fragments and scFv) in vitro. The display library which is normally expressed in phage or yeast can then be analyzed to select the desired trait resulting from the change in the sequence of the antibody.
How do you choose those with the antibodies you want? You can do this through a process called panning. One of the simplest breading methods is a variation of the common ELISA method.
To do this, incubate the antibody library immobilized on solids for the purpose. Any unbound phage was removed by washing and the bound phage was specifically eluted and amplified by infecting E. coli cells.